Immediately a drop of the reaction was transferred to the spot plate and 1 drop of I2KI was added. At this optimum temperature, the enzyme is most active and hence, takes less time to digest the starch.
Teaching notes This is a straightforward practical giving reliable, unambiguous results. Finally, the effects of enzyme concentration were tested.
Cite this Simulator:. If the reaction is too slow, increase the enzyme volume or concentration or reduce the starch volume or concentration.
One drop of each mixture was immediately transferred to the spot plate and a drop of I2KI was added. The optimum temperature means that the temperature at which the enzyme shows the maximum activity.
If we add saliva on starch, the salivary amylase present in saliva gradually acts on starch and converts it into maltose. Figure 3. New York: McGraw Hill, Above and below this range, the reaction rate reduces as enzymes get denaturated. The activity of enzymes is strongly affected by several factors, such as temperature and pH.
Mix using a plastic pipette.
Temperature seemed to have a positive correlations with the speed of the reaction.